NOTE: Please refer to primary antibody product webpage for recommended antibody dilution. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. For western blots, incubate membrane with diluted primary antibody in 5 w/v BSA, 1X TBS, 0.1 Tween 20 at 4C with gentle shaking, overnight. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.Īctin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. In contrast, filament nucleation by the Arp2/3 complex is not affected. Actin, cytoplasmic 1, N-terminally processed: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery. Western blot: Rat brain, Rat heart, Rat kidney, Rat stomach and Rat ovary lysates stained with ARG62346 anti-beta Actin antibody BA3R at 1:1000 dilution. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Our Actin polyclonal, monoclonal and recombinant monoclonal antibodies are developed in Rabbit. These antibodies target Actin in Human, Mouse, Rat, Bovine and Chicken samples. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization. Antibodies that detect Actin can be used in several scientific applications, including Immunohistochemistry, Western Blot, Immunocytochemistry, ELISA and Flow Cytometry. MICAL1 and MICAL2 produce the (R)-S-oxide form. Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICA元) to form methionine sulfoxide promotes actin filament depolymerization.
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